|CASE-FINDING BY SPUTUM MICROSCOPY
|N Naganathan, DR Nagpaul & SS Nair: Proceed
29th Natl TB & Chest Dis Workers Conf & 9th Eastern Region
Conf of IUAT, New Delhi, 1974, 351-58.
The findings of two studies, (i) one on comparison
of Ziehl-Neelsen method of staining of acid fast bacilli with and
without alcohol decolourisation and use of Gabbet's Methylene blue
(in place of decolourisation and counter staining) and (ii) comparison
of two different types of Basic Fuchsin dye used in the preparation
of Carbol Fuchsin, have been presented. The first study has shown
that omission of alcohol decolourisation or the use of Gabbet's
Methylene Blue has not influenced the detection of positives, though
the latter has more often produced a non- satisfactory background.
The second study has brought out the fact that two types of Basic
Fuchsin are similar in every respect. However, the findings does
not rule out the possibility of a bad dye giving rise to poor results.
Need for conducting studies for simplifying the staining procedure
has been stressed.
|KEY WORDS: COST, SPUTUM MICROSCOPY, STAINING
METHODS, ZIEHL1-NEELSEN, CASE-FINDING.