|UTILITY OF PYRUVATE MEDIUM FOR ISOLATION OF M.BOVIS
AND M.TUBERCULOSIS RESISTANT TO INH
|N Naganathan & R Rajalakshmi: Indian J Med Res
1977, 66, 556-61.
A study was conducted to evaluate the usefulness
of pyruvate medium for isolation of M.bovis from human material
and additional yield of M. tuberculosis resistant to INH. Specimens
from both rural and urban populations were included for this study
in order to understand the problem in both the situations. There
were two studies in progress at the National Tuberculosis Institute
when pyruvate media slopes were introduced for culture purpose.
One study was an epidemiological survey; 2518 sputum specimens received
from 51 villages covering a population of about 32,300 were used.
The specimens were collected from persons aged 5 years and above
showing abnormal shadow on X-ray. The other study was conducted
in collaboration with the State Tuberculosis Centre, Bangalore;
1204 sputum specimens were received from out patients attending
the centre. In addition to LJ medium, pyruvate medium was used for
isolation purposes. Identification and sensitivity tests were done
on positive cultures as per routine. In all, 129 cultures of tubercle
bacilli were isolated from 2118 specimens belonging to study 1 and
398 from 1204 specimens belonging to study 2. The number of cultures
contaminated were 253 and 35 respectively. No M.bovis was
isolated in either study. There were 24 and 23 cultures resistant
to INH among those isolated from LJ and pyruvate medium respectively.
Thus, no increase was observed in the isolation of INH resistant
strains using pyruvate medium.
Hence, no benefit was derived by using this
medium over and above what was obtained from plain Lowenstein Jensen
medium in both the situations.
|KEY WORDS: PYRUVATE MEDIUM, LJ MEDIUM, M.TUBERCULOSIS,
DRUG RESISTANCE, M.BOVIS, RURAL POPULATION, URBAN POPULATION.