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146
CASE-FINDING BY SPUTUM MICROSCOPY
N Naganathan, DR Nagpaul & SS Nair: Proceed 29th Natl TB & Chest Dis Workers Conf & 9th Eastern Region Conf of IUAT, New Delhi, 1974, 351-58.

The findings of two studies, (i) one on comparison of Ziehl-Neelsen method of staining of acid fast bacilli with and without alcohol decolourisation and use of Gabbet's Methylene blue (in place of decolourisation and counter staining) and (ii) comparison of two different types of Basic Fuchsin dye used in the preparation of Carbol Fuchsin, have been presented. The first study has shown that omission of alcohol decolourisation or the use of Gabbet's Methylene Blue has not influenced the detection of positives, though the latter has more often produced a non- satisfactory background. The second study has brought out the fact that two types of Basic Fuchsin are similar in every respect. However, the findings does not rule out the possibility of a bad dye giving rise to poor results. Need for conducting studies for simplifying the staining procedure has been stressed.

KEY WORDS: COST, SPUTUM MICROSCOPY, STAINING METHODS, ZIEHL1-NEELSEN, CASE-FINDING.
 
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